Tag

Part:BBa_K4806006:Design

Designed by: Luca Langenberg   Group: iGEM23_RPTU-Kaiserslautern   (2023-09-15)


mNeonGreen-tag for Chlamydomonas reinhardtii (Phytobrick)


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 311
    Illegal PstI site found at 718
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 311
    Illegal PstI site found at 718
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 327
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 311
    Illegal PstI site found at 718
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 311
    Illegal PstI site found at 718
    Illegal AgeI site found at 656
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part is codon optimized for Chlamydomonas reinhardtii and compatible with Phytobricks/MoClo parts.

Source

This part was built by our department of biotechnology and systemsbiology from the RPTU Kaiserslautern. Its original host organism is Branchiostoma lanceolatum
Source: https://www.uniprot.org/uniprotkb/A0A1S4NYF2/entry

References